Elicitation of Isatis tinctoria L. hairy root cultures by salicylic acid and methyl jasmonate for the enhanced production of pharmacologically active alkaloids and flavonoids
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چکیده
منابع مشابه
Effect of elicitation on antioxidant activity and production of tropane alkaloids in Hyoscyamus reticulatus hairy root cultures
Background and objectives: Hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. In the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in Hyoscyamus reticulatus. M...
متن کاملEstablishment of Hairy Root Cultures by Agrobacterium Rhizogenes Mediated Transformation of Isatis Tinctoria L. for the Efficient Production of Flavonoids and Evaluation of Antioxidant Activities
In this work, Isatis tinctoria hairy root cultures (ITHRCs) were established as an alternative source for flavonoids (FL) production. I. tinctoria hairy root line V was found to be the most efficient line and was further confirmed by the PCR amplification of rolB, rolC and aux1 genes. Culture parameters of ITHRCs were optimized by Box-Behnken design (BBD), and eight bioactive FL constituents (r...
متن کاملBiotic elicitation for scopolamine production by hairy root cultures of Datura metel
The (-)-hyoscyamine, atropine and scopolamine (hyoscine) are three valuable tropane alkaloids while scopolamine is the most important member of this group for the pharmaceutical industry due to its higher demand compared to hyoscyamine and atropine. Scopolamine is an anticholinergic reagent with several therapeutic applications. In the current study, the hairy roots culture of Daturametel was u...
متن کاملGenista tinctoria hairy root cultures for selective production of isoliquiritigenin.
Hairy root cultures were established after inoculation of Genista tinctoria in vitro shoots with Agrobacterium rhizogenes, strain ATCC 15834. In transformed roots of G. tinctoria grown in Schenk-Hildebrandt medium without growth regulators the biosynthesis of isoflavones, derivatives of genistein and daidzein, and flavones, derivatives of luteolin and apigenin, characteristic for the intact pla...
متن کاملEnhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum
Background: Flavonoids of Hypericum perforatum are important secondary metabolites which have been widely utilized in medicine for a range of purposes. The use of methyl jasmonate (MeJA) elicitation for the enhancement of flavonoid production in cell suspension culture of H. perforatum would be an efficient alternative method for the flavonoid production. Results: MeJA influenced the cells grow...
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ژورنال
عنوان ژورنال: Plant Cell, Tissue and Organ Culture (PCTOC)
سال: 2019
ISSN: 0167-6857,1573-5044
DOI: 10.1007/s11240-018-01553-8